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The pigment is applied externally at concentrations between 0.01 percent and 0.1 percent in creams, gels, or solutions designed for dermatological use

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For the MTT assay, HT-22 cells were seeded into 96-well, flat-bottomed plates at a density of 3000 cells/well in 100 µl DMEM (high glucose, 1 mM pyruvate, 10% FBS) and allowed to attach overnight